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KMID : 0379120100380020172
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Korean Journal of Mycology
2010 Volume.38 No. 2 p.172 ~ p.178
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Physicochemical Properties and Physiological Activities of Rhus verniciflua Stem Bark Cultured with Fomitella fraxinea
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Choi Han-Seok
Kim Bo-Hyun
Yeo Soo-Hwan
Jeong Seok-Tae
Choi Ji-Ho
Park Hyo-Suk
Kim Myung-Gon
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Abstract
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The contents of proximate composition, free amino acids and phenolic acids in the Fomitella fraxinea cultivated-Rhus verniciflua stem bark(FRVSB), and its adipogenesis effect were investigated. The proximate composition(%) of FRVSB was as follows: moisture(7.64), ash(6.30), crude fat(3.86), crude protein(3.59) and sugar(not detected); while Rhus verniciflua stem bark(RVSB) contained 1.64, 8.09, 7.28, 6.48 and 5.39, respectively. The total free amino acids concentration was 97.41 mg% in FRVSB and 71.91 mg% in RVSB. Phosphoserine(55.06 mg%), ammonia(17.84mg%) and aspartic acid(6.05mg%) were predominant amino acids. The content of total phenolic acids was 422.89 ppm in ethanol extract and 283.86 ppm in water extract, with syringic and gallic acid as the main component. The FRVSB extracts showed a potent free radical scavenging activity for DPPH(2,2-diphenyl-1-picrylhydrazyl hydrate) with of (EtOH) and (water), respectively, whereas value of gallic acid was . The protective effect of both ethanol and water extract the extracts against UV-induced oxidative stress in NIH3T3 was observed. The water extracts of FRVSB may promote adipogenesis in 3T3-L1 cells.
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KEYWORD
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Adipogenesis, Anti-oxidant activity, Fomitella fraxinea, Proximate composition, Rhus verniciflua
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